His-Tag Polyclonal Antibody | E-AB-20001

His-Tag Polyclonal Antibody | E-AB-20001 | Gentaur US, UK & Europe Disrtribition

Reactivity: N/A

Host: Rabbit

Isotype: IgG

Application: WB, ELISA

Research Area: Tags and Cell Markers

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: 6X His synthetic peptide conjugated to KLH.

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: WB 1:1000-1:3000, ELISA 1:20000

Conjugation: Unconjugated

Calculated Molecular Weight: N/A

Observed Molecular Weight: 15 kDa

Clonality: Polyclonal