HDAC Assay Buffer | 50031

HDAC Assay Buffer | 50031 | Gentaur US, UK & Europe Disrtribition

Category: Deacetylase/Assay Kit

Application: Suitable for use with cell extracts, nuclear extracts, immunoprecipitates, and purified HDAC enzymes.

Background: N/A

Description: HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.

Product Type: Buffer

Shippement Condition: -20°C