Description
FUGENE® 4K TRANSFECTION REAGENT
Best-in-Class DNA Transfection Reagent
FuGENE® 4K is our newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into both challenging and routine mammalian cell lines. FuGENE® 4K was developed to improve upon our widely published and trusted FuGENE® HD reagent. Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE® 4K provides efficient and safe entry of DNA into a wide range of cells. This mechanism allows users to obtain higher performance than other competitor reagents including Lipofectamine® 3000.
Key Features
- Outperforms Lipofectamine® 3000 in most cell types
- Most effective broad spectrum DNA transfection reagent
- Maximum transfection efficiency in adherent cells
- Robust performance in suspension adapted 293 & CHO cells
- Improved viral titers and protein production yields.
Benefits of FuGENE® 4K
- Superior transfection efficiencies compared to competitors
- Increased performance when compared to FuGENE® HD
- Transfect in serum-free or serum-containing media.
- Quick and easy protocol with limited optimization steps
- Transfect difficult-to-transfect mammalian cell lines
- Improved performance in the most relevant cancer cell lines
- Improved viral titer and capsid fullness in AAV/LV systems
- Maximizes protein production in 293 & CHO cells
FuGENE® 4K vs. Lipofectamine® 3000
FuGENE® 4K reagent outperforms Lipofectamine® 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.
Benefits of FuGENE® 4K vs Lipofectamine® 3000
- PERFORMANCE: Obtain higher transfection efficiencies with FuGENE® 4K compared to Lipofectamine® 3000.
- EASE OF USE: FuGENE® 4K is a single reagent kit, while Lipofectamine® requires addition of separate reagent.
- CELL VIABILITY: FuGENE® 4K has comparable cell viability as Lipofectamine® across most cell lines.
- FASTER PROTOCOL: FuGENE® 4K forms complex with DNA in as little as 5 minutes, and does not require additional component added to the transfection mix which requires preparation of an additional tube.
- COST SAVINGS: Less cost per mL of FuGENE® 4K vs. Lipofectamine® 3000. (at smallest available vial sizes).
Protocol
