Flag-Tag Polyclonal Antibody | E-AB-40501

Flag-Tag Polyclonal Antibody | E-AB-40501 | Gentaur US, UK & Europe Disrtribition

Reactivity: Others

Host: Rabbit

Isotype: IgG

Application: WB

Research Area: Tags and Cell Markers

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.

Lead Time: 7~10 days

Concentration: 0.93mg/mL

Storage: Store at -20℃. Avoid freeze / thaw cycles.

Immunogen: Synthetic peptide

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.

Purification: Antigen Affinity Purification

Dilution: WB 1:500-1:2000

Conjugation: Unconjugated

Calculated Molecular Weight: 35 kDa

Observed Molecular Weight: 35 kDa

Clonality: Polyclonal