DU4475 Cell Line | CL-0719

DU4475 Cell Line | CL-0719 | Gentaur US, UK & Europe Disrtribition

Media: CM-0719

Organism: Homo sapiens, Human

Application: N/A

Background: established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described in the literature to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and as triple-negative

Age: 62Y

Tissue: Breast; Mammary gland

Morphology: epithelial

Growth Properties: Suspension, multicellular aggregates

Doubling Time: 80-100 hours

Biosafty: 1

Medium: RPMI-1640 (PM150110)+10% FBS+1% P/S (PB180120)

Subculturing: N/A

Ratio: 1:2 to 1:3

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+72% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: Yes, in nude mice

Duration: N/A

Lead Time: 3-5 days