Description
DU4475 Cell Line | CL-0719 | Gentaur US, UK & Europe Disrtribition
Media: CM-0719
Organism: Homo sapiens, Human
Application: N/A
Background: established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described in the literature to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and as triple-negative
Age: 62Y
Tissue: Breast; Mammary gland
Morphology: epithelial
Growth Properties: Suspension, multicellular aggregates
Doubling Time: 80-100 hours
Biosafty: 1
Medium: RPMI-1640 (PM150110)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: 1:2 to 1:3
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+72% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: Yes, in nude mice
Duration: N/A
Lead Time: 3-5 days
