DB cell line | CL-0645

DB cell line | CL-0645 | Gentaur US, UK & Europe Disrtribition

Media: CM-0645

Organism: Homo sapiens, human

Application: N/A

Background: The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma

Age: Male,45 years

Tissue: Ascites

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: ~50 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:5

Renewal: Every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage Temperature: Liquid Nitrogen Vapor Phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days