Description
DB cell line | CL-0645 | Gentaur US, UK & Europe Disrtribition
Media: CM-0645
Organism: Homo sapiens, human
Application: N/A
Background: The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma
Age: Male,45 years
Tissue: Ascites
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: ~50 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
