CD20 Antibody | V3727SAF-100UG

CD20 Antibody | V3727SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: rIGEL/773

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IF, FACS, IHC-P

Application Details: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein G affinity chromatography

Description: Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This mAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Immunogen: Human CD20 protein was used as the immunogen for this recombinant CD20 antibody.

Storage: Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20 °C or colder.

Localization: Predominantly cell surface with some cytoplasmic