Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified) | H2A-M82E3-200ug

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified) | H2A-M82E3-200ug| Gentaur Distribution US, UK & Europe

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.

Source: Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (H2A-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (B2M) & Gly 22 - Met 305 (H-2Kb) & SIINFEKL peptide (Accession # P01887 (B2M) & P01901 (H-2Kb) & SIINFEKL).

Format: Powder.

Tag: C-10×His & C-Avi.

Expression System: HEK293.

Expression Region: Ile 21 - Met 119 (B2M) & Gly 22 - Met 305 (H-2Kb) & SIINFEKL peptide.

Conjugate: Biotin-labeled.

Molecular Weight: 49.1 kDa.

Characteristics: Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 49.1 kDa. The protein migrates as 55-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: PBS, pH7.4.

Storage Conditions: -20℃.

Shipping Conditions: RT