beta actin Polyclonal Antibody | E-AB-40338

beta actin Polyclonal Antibody | E-AB-40338 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Application: WB, IHC

Research Area: Isotype, Tags & Cell Markers, Signal Transduction

Background: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Lead Time: 7~10 days

Concentration: 0.85 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant Zebrafish Actin, cytoplasmic 2 protein

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3

Purification: Antigen Affinity Purification

Dilution: WB 1:1000-1:2000 IHC 1:100-1:300

Conjugation: Unconjugated

Calculated Molecular Weight: 41 kDa

Observed Molecular Weight: 41 kDa

Clonality: Polyclonal