B2M (Human) CRISPR/Cas9 Lentivirus (Integrating) | 78340

B2M (Human) CRISPR/Cas9 Lentivirus (Integrating) | 78340 | Gentaur US, UK & Europe Disrtribition

Category: CRISPR/Lentivirus

Application: 1. Transient knock-down of B2M in target cells
2. Generation of a stable B2M knock-out cell pool following puromycin selection and limited dilution

Background: N/A

Description: Beta-2 Microglobulin (B2M) is a required component of Major Histocompatibility Complex (MHC) class 1 molecules, which present peptide fragments from within the cell to cytotoxic T-cells as part of the adaptive immune system. The B2M CRISPR/Cas9 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNAs) targeting human B2M driven by a U6 promoter. The integrating lentivirus integrates randomly into the cellular genome to express both Cas9 and the sgRNA. Puromycin selection forces high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies will depend on the cell type and the gene of interest.

Product Type: Lentivirus

Shippement Condition: -80°C