AAV9 Luciferase-eGFP | 78468

AAV9 Luciferase-eGFP | 78468 | Gentaur US, UK & Europe Disrtribition

Category: Viral Tools/AAV

Application: Use as a positive control for transduction Optimize transduction assays and track protein expression over time

Background: N/A

Description: Adeno-Associated Virus serotype 9 (AAV9) is one of the most promising serotypes for gene therapy applications. AAV9 transduces a wide range of tissue types, including cardiac and skeletal muscle, liver, pancreas, and eye tissue. AAV8 and AAV9 have recently been used to correct disease-causing mutations and improve muscle function in mouse models of Duchenne muscular dystrophy. AAV9 has significantly lower seroprevalence in the human population than other AAV serotypes, making it a desirable candidate for therapeutic applications. These AAV particles constitutively express the firefly (Photinus pyralis) luciferase and enhanced Green Fluorescent Protein (eGFP) genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript and expression of luciferase and eGFP as two separate proteins.

Product Type: Viral Tools

Shippement Condition: -80°C