Description
Y3-Ag 1.2.3 cell line | CL-0297 | Gentaur US, UK & Europe Disrtribition
Media: CM-0297
Organism: Rattus norvegicus, Rat
Application: N/A
Background: This cell line is derived from an azaguanine-resistant mutant of S210 myeloma. The cells are resistant to 8-azaguanine but sensitive to HAT. The cells can be used as a rat B cell fusion partner for preparing rat-rat hybridomas. Commercial use or third party distribution has to be permitted by C. Milstein.
Age: N/A
Tissue: Disease: Plaemocytoma; Myeloma Strain: Lou; Cell Type: B Lymphoblastoma
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1×10^5 to 1×10^6 cells/mL
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
