WT1 Antibody / Wilms Tumor 1 | V9173-100UG

WT1 Antibody / Wilms Tumor 1 | V9173-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: rWT1/6908

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml

Application Note: Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.

Purity: Protein A/G affinity

Description: The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus. In tumor tissues, WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas. WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from non-serous carcinomas.

Immunogen: A portion of amino acids 1-100 was used as the immunogen for the recombinant WT1 antibody.

Storage: Aliquot the recombinant WT1 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.

Localization: Nucleus and cytoplasm