Description
WPMY-1 Cell Line | CL-0467 | Gentaur US, UK & Europe Disrtribition
Media: CM-0467
Organism: Homo sapiens, Human
Application: Because of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal(epithelial interactions).
Background: The depositor reports that the RWPE-1 cell line, derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Age: 54 years
Tissue: Prostate/stroma
Morphology: Myofibroblast
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2
Medium: DMEM(PM150210)+5% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: No, in nude mice(at Passage 22 cells did not form tumors when injected subcutaneously); Yes, the cells form colonies in soft agar(colony forming efficiency of 0.7%).
Duration: N/A
Lead Time: 3-5 days
