Vimentin Antibody | V9292-100UG

Vimentin Antibody | V9292-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: rVIM/6431

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human (broad species reactivity predicted)

Application: WB, IHC-P

Application Details: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

Application Note: Optimal dilution of the recombinant VIM antibody should be determined by the researcher.

Purity: Protein A/G affinity

Description: Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant VIM antibody.

Storage: Aliquot the recombinant VIM antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.

Localization: Cytoplasmic