Description
Vimentin Antibody | V9284-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: VIM/6430R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human, Mouse, Rat, Dog, Cat, Cow, Horse
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
Storage: Aliquot the recombinant Vimentin antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Cytoplasmic
