Description
Ubiquitin-Rhodamine | 81151 | Gentaur US, UK & Europe Disrtribition
Category: Ubiquitination/Deubiquitylating Enzymes
Application: Useful for assaying ubiquitin C-terminal hydrolytic activity, for studying the activity and specificity of ubiquitin and ubiquitin-like hydrolases, and for screening for modulators of deubiquitylase activity.
Background: While the disubstituted rhodamine moiety in Ub-Rho110-G is essentially non-fluorescent, cleavage results in a mono-substituted rhodamine, Rho110-G, which exhibits intense fluorescence when excited at 485 nm. The longer excitation and emission wavelengths (Ex485 nm, Em535 nm) of the rhodamine fluorophore reduces the risk of artifacts in screens due to autofluorescence, which can make ubiquitin-rhodamine more appropriate than Ubiquitin-AMC for some compound screening and profiling assays.
Description: Ubiquitin-rhodamine 110 is a quenched, fluorescent substrate for deubiquitylases, especially ubiquitin C-terminal hydrolases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
Product Type: Protein
Shippement Condition: -80°C
