Description
Ubiquitin AMC | 81150 | Gentaur US, UK & Europe Disrtribition
Category: Ubiquitination/Deubiquitylating Enzymes
Application: Useful as a substrate for deubiquitinating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs), as well as investigation of deconjugating enzyme substrate specificity and for screening for modulators of
Background: Ubiquitin-AMC is prepared via the conjugation of the C-terminus of mature, Ubiquitin with 7-amino-4-methylcoumarin (AMC). This conjugation quenches the intrinsic fluorescence of AMC. The high purity of BPS's Ubiquitin-AMC compared to that of other commercial vendors leads to a greater signal:background.
Description: Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.
Product Type: Protein
Shippement Condition: -80°C
