TT Cell Line | CL-0457

TT Cell Line | CL-0457 | Gentaur US, UK & Europe Disrtribition

Media: CM-0457

Organism: Homo sapiens, Human

Application: N/A

Background: TT cells continuously produce high levels of calcitonin and CEA.Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively, after a medium change.CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period.Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1mM L-glutamine.It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F-12K medium.Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.

Age: 77 years

Tissue: Thyroid/medulla

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: Ham's F-12K(PM150910)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.

Duration: N/A

Lead Time: 3-5 days