TNF alpha Antibody | V2902-20UG

TNF alpha Antibody | V2902-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: J2D10

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: FACS, IF, Inhibition, Neutralization

Buffer: N/A

Limitation: This TNF-alpha antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: This antibody neutralises human TNFa mediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of hTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Immunogen: Recombinant human protein was used as the immunogen for the TNF-alpha antibody.

Storage: Store the TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).