Description
Thymidylate Synthase Antibody | V8144-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rTYMS/1884
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: FACS, IF
Application Details: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
Application Note: Optimal dilution of the antibody should be determined by the researcher.
Purity: Protein G affinity chromatography
Description: It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Immunogen: A recombinant human partial protein (amino acids 60-174) was used as the immunogen for the recombinant Thymidylate Synthase antibody.
Storage: Store the recombinant Thymidylate Synthase antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Nuclear, cytoplasmic
