Thymidine Phosphorylase Antibody / PD-ECGF | V8507SAF-100UG

Thymidine Phosphorylase Antibody / PD-ECGF | V8507SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: rTYMP/3444

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P, WB

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 1-2ug/ml

Application Note: Optimal dilution of the recombinant PD-ECGF antibody should be determined by the researcher.

Purity: Protein G affinity chromatography

Description: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant PD-ECGF antibody.

Storage: Store the recombinant PD-ECGF antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cytoplasmic, nuclear