Thrombomodulin Antibody / CD141 | V3557-100UG

Thrombomodulin Antibody / CD141 | V3557-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: rTHBD/1591

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: ELISA, IHC-P

Application Details: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Thrombomodulin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein G affinity chromatography

Description: It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this recombinant Thrombomodulin antibody.

Storage: Store the recombinant Thrombomodulin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface, cytoplasmic