Description
TF-1 Cell Line | CL-0232 | Gentaur US, UK & Europe Disrtribition
Media: CM-0232
Organism: Homo sapiens, Human
Application: N/A
Background: N/A
Age: 35 years
Tissue: Peripheral blood
Morphology: Lymphoblast
Growth Properties: Mixed, Adherent And Suspension
Doubling Time: N/A
Biosafty: 1
Medium: RPMI-1640(PM150110)+2ng/ml rhGM-CSF+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
