Description
TDO Cellular Activity QuickDetect™ Supplements | 62002-1 | Gentaur US, UK & Europe Disrtribition
Category: Immunotherapy/Cell Line
Application: • Monitor TDO pathway activity • Screen for activators or inhibitors of TDO in a cellular context
Background: L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. TDO is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description: The TDO Cellular Activity QuickDetectTM Supplements are designed to complement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Product Type: Media & Reagents
Shippement Condition: dry ice
