Description
T4 DNA Ligase | G467| Gentaur Distribution US, UK & Europe
Product Description: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Application: • Cloning of restriction fragments • Attaching double-stranded oligonucleotide linkers or adaptors to DNA • Site-directed mutagenesis • Nick repair of duplex DNA, RNA or DNA-RNA hybrids • Self-circularization of linear DNA
Shipping Conditions: Dry Ice
Storage Conditions: Store all components at -20°C.
