SW480 [SW-480] Cell Line | CL-0223

SW480 [SW-480] Cell Line | CL-0223 | Gentaur US, UK & Europe Disrtribition

Media: CM-0223

Organism: Homo sapiens, Human

Application: This cell line is a suitable transfection host. This line has a mutation in codon 12 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.

Background: A cell line established from a lymph node metastasis taken from the same patient one year later is available.The line is negative for CSAp(CSAp-) and colon antigen 3.The cells are positive for keratin by immunoperoxidase staining.There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution and a C-】 T mutation in codon 309 resulting in a Pro-】 Ser substitution.The cells express elevated levels of p53 protein.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.N-myc oncogene expression was not detected.Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed.The cells have been reported to produce GM-CSF.

Age: 50 years

Tissue: Colon

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: Leibovitz's L-15(PM151010)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice.

Duration: N/A

Lead Time: 3-5 days