Description
SW1116 Cell Line | CL-0307 | Gentaur US, UK & Europe Disrtribition
Media: CM-0307
Organism: Homo sapiens, Human
Application: N/A
Background: CSAp negative(CSAp-).Colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.N-myc and N-ras oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Age: 73 years
Tissue: Colon
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: Leibovitz's L-15(PM151010)+10% FBS+1% P/S(PB180120)
Subculturing: Cells must be subcultured at about 80% confuency, before they reach 90%. Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
Duration: N/A
Lead Time: 3-5 days
