SV40 MES 13 Cell Line | CL-0470

SV40 MES 13 Cell Line | CL-0470 | Gentaur US, UK & Europe Disrtribition

Media: CM-0470

Organism: Mus musculus, Mouse

Application: N/A

Background: The cells form colonies in soft agar, and are positive for SV40 large T antigen.The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1×10^-6 M angiotensin Ⅱ. The cells form colonies in soft agar, and are positive for SV40 large T antigen.

Age: 7 to 10 weeks

Tissue: Kidney/glomerulus

Morphology: Myofibroblast-like

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 2

Medium: 71.25% DMEM(PM150210)+23.75% Ham's F-12(PM150810)+5% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, forms colonies in soft agar.

Duration: N/A

Lead Time: 3-5 days