Description
Super Tube cell line | CL-0473 | Gentaur US, UK & Europe Disrtribition
Media: CM-0473
Organism: Canis familiaris
Application: N/A
Background: Super Tube is an epothelial-like cell line cloned from the MDCK, a cell line from an adult cocker spaniel, Canis familiaris. Compared to the original cell line, Super Tube expresses a much lower cyclic-AMP resting level and responces less readily to forskolin stimulation of adenylate cyclase. Additionally, Super Tube shows a lower transepithelal resistance compared to Super Dome and MDCK.
Age: female, adult
Tissue: Normal Kidney
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: DMEM/F12(PM150312)+0.05mM NEAA+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
