Description
SRE eGPF Reporter - HEK293 Cell Line (ERK Pathway) | 78327 | Gentaur US, UK & Europe Disrtribition
Category: Cell Signaling/Cell Line
Application: • Screen for compound effect on the MAPK/ERK signaling pathway.
• Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
Background: The MAPK/ERK signaling pathway is a key regulator of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The Ternary Complex Factors (TCFs), including Elk1, are among the best-characterized transcription factor substrates of ERK1/2. When phosphorylated by ERK1/2, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
Description: Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway.
Product Type: Cell Line
Shippement Condition: -80°C
