SPTBN2 Antibody / Spectrin beta III | V7461-20UG

SPTBN2 Antibody / Spectrin beta III | V7461-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: SPTBN2/2979R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: WB, IHC-P

Application Details: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.

Storage: Store the recombinant Spectrin beta III antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface, cytoplasm