Description
Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit | 78357 | Gentaur US, UK & Europe Disrtribition
Category: Coronavirus/Assay Kit
Application: This kit is useful for screening for inhibitors of ACE2 binding to the RBD domain of SARS-CoV-2 Spike, Omicron variant.
Background: The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and has recently become a global variant of concern. Drugs targeting the interaction between SARS-CoV-2 Spike protein and human ACE2 may offer some protection against viral infection. The SARS-CoV-2 Spike RBD (B.1.1.529 BA.1, Omicron Variant) protein in this kit consists of the RBD region of the S1 protein, which includes the ACE2 binding site. The omicron variant of SARS-CoV-2 has at least 3 sublineages, BA.1., BA.2, and BA.3. The protein contains all the mutations observed in this region of the omicron B.1.1.529 BA.1 variant compared to the wild-type strain.
Description: The Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling neutralizing antibodies or inhibitors of the interaction between the Omicron variant SARS-CoV-2 Spike RBD and human ACE2. This kit comes in a convenient 96-well format, with Biotinylated-ACE2, purified Spike RBD (Omicron Variant; B.1.1.529 BA.1) protein, Streptavidin-HRP, and assay buffers for 100 reactions. The assay requires only a few steps. First, SARS-CoV-2 Spike RBD (Omicron Variant; B.1.1.529 BA.1) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a chemiluminescence HRP substrate to produce the luminescence signal.
Product Type: Assay Kit
Shippement Condition: -80°C
