Description
Spike S1 (B.1.617.1; Kappa Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit | 78283 | Gentaur US, UK & Europe Disrtribition
Category: Coronavirus/Assay Kit
Application: This kit is useful for screening for inhibitors of the interaction between SARS-CoV-2 Spike S1 (B.1.617.1) and human ACE2.
Background: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection. A variant called B.1.617.1 (also known as the Kappa variant) was identified in India in the spring of 2021. This variant has a number of mutations in the Spike protein (G142D, E154K, L452R, E484Q, D614G, P681R) that may allow the virus to spread more easily and quickly than other variants.
Description: The Spike S1 (B.1.617.1; Kappa Variant) (SARS-CoV-2): ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between Spike S1 (B.1.617.1) (SARS-CoV-2) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and the dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).
Product Type: Assay Kit
Shippement Condition: -80°C
