Description
Spike S1 (B.1.1.7; Alpha Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit | 78282 | Gentaur US, UK & Europe Disrtribition
Category: Coronavirus/Assay Kit
Application: This kit is useful for screening for inhibitors of the interaction between SARS-CoV-2 Spike S1 (B.1.1.7; Alpha Variant) and human ACE2.
Background: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection. SARS-CoV-2 (B.1.1.7), also known as Alpha variant, was first identified in the United Kingdom in the summer of 2020. This variant contains mutations N501Y, A570D, D614G, P681H, and deletions 69-70HV and 144Y in the Spike protein that may lead to higher transmissibility and infectivity.
Description: The Spike S1 (B.1.1.7; Alpha Variant) (SARS-CoV-2): ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (B.1.1.7; Alpha Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).
Product Type: Assay Kit
Shippement Condition: -80°C
