Description
SNAP25 Polyclonal Antibody | E-AB-40191 | Gentaur US, UK & Europe Disrtribition
Reactivity: Mouse
Host: Rabbit
Isotype: IgG
Application: WB
Research Area: Cancer, Metabolism, Neuroscience, Signal Transduction
Background: The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
Lead Time: 7~10 days
Concentration: 2 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Recombinant Mouse Synaptosomal-associated protein 25 protein
Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification: Affinity purification
Dilution: WB 1:500-1:2000
Conjugation: Unconjugated
Calculated Molecular Weight: 23 kDa
Observed Molecular Weight: 23 kDa
Clonality: Polyclonal
