SMAD3 Polyclonal Antibody | E-AB-40050

SMAD3 Polyclonal Antibody | E-AB-40050 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Rat

Host: Rabbit

Isotype: IgG

Application: WB, IHC

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Lead Time: 7~10 days

Concentration: 2 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant human Mothers against decapentaplegic homolog 3 protein

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Purification: Affinity purification

Dilution: WB 1:500-1:1000, IHC 1:50-1:100

Conjugation: Unconjugated

Calculated Molecular Weight: 48 kDa

Observed Molecular Weight: 58 kDa

Clonality: Polyclonal