SK-NEP-1 Cell Line | CL-0442

SK-NEP-1 Cell Line | CL-0442 | Gentaur US, UK & Europe Disrtribition

Media: CM-0442

Organism: Homo sapiens, Human

Application: N/A

Background: Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles.

Age: 25 years

Tissue: Kidney, derived from metastatic site: pleural effusion

Morphology: Rounded

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: McCoy’s 5A(PM150710)+15% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:6.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice forms tumor with small cells consistent with Wilms'tumor.

Duration: N/A

Lead Time: 3-5 days