SK-N-BE(2) Cell Line | CL-0441

SK-N-BE(2) Cell Line | CL-0441 | Gentaur US, UK & Europe Disrtribition

Media: CM-0441

Organism: Homo sapiens, Human

Application: N/A

Background: The cells exhibit moderate levels of dopamine beta hydroxylase activity.SK-N-BE(2) cells have a reported saturation density greater than 1×10^6 cells/cm^2.The morphology of the cells varies with some cells having long processes and others that are epithelioid like.The cells will aggregate, form clumps and float.

Age: 2 years

Tissue: Brain, derived from metastatic site: bone marrow

Morphology: Neuroblast

Growth Properties: Mixed, Adherent And Suspension

Doubling Time: N/A

Biosafty: 1

Medium: MEM/F12+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, an inoculum of 10^7 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency.

Duration: N/A

Lead Time: 3-5 days