Description
SK-MEL-1 Cell Line | CL-0440 | Gentaur US, UK & Europe Disrtribition
Media: CM-0440
Organism: Homo sapiens, Human
Application: Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Background: Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Age: 29 years
Tissue: Malignant melanoma, skin, derived from metastatic site: lymphatic system
Morphology: Spherical
Growth Properties: Clusters In Suspension
Doubling Time: N/A
Biosafty: 1
Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in nude mice forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters.
Duration: N/A
Lead Time: 3-5 days
