SK-MEL-1 Cell Line | CL-0440

SK-MEL-1 Cell Line | CL-0440 | Gentaur US, UK & Europe Disrtribition

Media: CM-0440

Organism: Homo sapiens, Human

Application: Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.

Background: Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.

Age: 29 years

Tissue: Malignant melanoma, skin, derived from metastatic site: lymphatic system

Morphology: Spherical

Growth Properties: Clusters In Suspension

Doubling Time: N/A

Biosafty: 1

Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters.

Duration: N/A

Lead Time: 3-5 days