Description
SHZ-88 cell line | CL-0209 | Gentaur US, UK & Europe Disrtribition
Media: CM-0209
Organism: Rattus norvegicus, Rat
Application: N/A
Background: The SHZ-88 cell line is derived from a breast cancer of DMBA-induced rat. SHZ-88 cells are mostly epithelial-like cells, with large cell clusters and pathological mitotic ones can be observed. Microfilaments on the surface of cells and on the desmosome can be observed by electron microscopy. The mitotic index of the cells on day 3 is 59% and the cells can survive after homologous animal transplantation.
Age: N/A
Tissue: Mastocarcincma
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: N/A
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: N/A
Renewal: N/A
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
