SH-SY5Y [SHSY-5Y] Cell Line | CL-0208

SH-SY5Y [SHSY-5Y] Cell Line | CL-0208 | Gentaur US, UK & Europe Disrtribition

Media: CM-0208

Organism: Homo sapiens, Human

Application: Transfection host.

Background: SH-SY5Y cells have a reported saturation density greater than 1×10^6 cells/cm^2. They are reported to exhibit moderate levels of dopamine beta hydroxylase activity. Ref

Age: 4 years

Tissue: Bone marrow

Morphology: Epithelial-like and neuronal-like

Growth Properties: Mixed, Adherent And Suspension

Doubling Time: ~60-90 hours

Biosafty: 1

Medium: MEM/F12(PM151220)+15% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days