Description
Sf9 Cell Line | CL-0205 | Gentaur US, UK & Europe Disrtribition
Media: CM-0205
Organism: Spodoptera frugiperda, Fall armyworm
Application: N/A
Background: N/A
Age: Pupa
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Mixed, adherent and suspension
Doubling Time: ~27-50 hours
Biosafty: 1
Medium: TNM-FH(PM152010)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 2×10^5 to 5×10^5cells/mL
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+14% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 100%. Temperature: 28℃.
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
