Sf9 Cell Line | CL-0205

Sf9 Cell Line | CL-0205 | Gentaur US, UK & Europe Disrtribition

Media: CM-0205

Organism: Spodoptera frugiperda, Fall armyworm

Application: N/A

Background: N/A

Age: Pupa

Tissue: Ovary

Morphology: Epithelial

Growth Properties: Mixed, adherent and suspension

Doubling Time: ~27-50 hours

Biosafty: 1

Medium: TNM-FH(PM152010)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Ratio: 2×10^5 to 5×10^5cells/mL

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+14% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 100%. Temperature: 28℃.

Effects: N/A

Duration: N/A

Lead Time: 3-5 days