SBE Reporter Kit (TGFβ/SMAD signaling pathway) | 60654

SBE Reporter Kit (TGFβ/SMAD signaling pathway) | 60654 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Cell Based Assay Kit/Cell Signaling Pathway

Application: • Monitor TGFβ signaling pathway activity.
• Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.

Background: N/A

Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

Product Type: Reporter Assay Kit

Shippement Condition: -20°C