SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway) | 79806

SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway) | 79806 | Gentaur US, UK & Europe Disrtribition

Category: Cell Line/Lentiviruses

Application: • Screen for activators or inhibitors of the TGFβ/SMAD signaling pathway in transduced target cells
• Generation of SBE Luciferase Reporter stable cell line

Background: The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer's and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/SMAD responsive genes.

Description: The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity.

Product Type: Lentivirus

Shippement Condition: -80°C