Description
SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag | NUN-C52Hg-100ug| Gentaur Distribution US, UK & Europe
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Source: SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag (NUN-C52Hg) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R) ). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR) -linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.
Format: Powder.
Tag: C-6×His.
Expression System: HEK293.
Expression Region: Met 1 - Ala 419.
Conjugate: Unconjugated.
Molecular Weight: 47.4 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 90%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
