Description
SARS-CoV-2 Nucleocapsid protein (P80R), His Tag | NUN-C52Hc-1mg| Gentaur Distribution US, UK & Europe
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Source: SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (NUN-C52Hc) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P80R) ). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR) -linker responsible for phosphorylation. The mutation P80R was identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3) (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
Format: Powder.
Tag: C-6×His.
Expression System: HEK293.
Expression Region: Met 1 - Ala 419.
Conjugate: Unconjugated.
Molecular Weight: 47.4 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.4 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: PBS, 0.5 M Arginine, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
