Description
SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit | E-EL-E601 | Gentaur US, UK & Europe Disrtribition
Target Species: Human
Type: Indirect
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivitys: /
Detection Range: /
Target Name: SARS-CoV-2 Nucleocapsid Protein IgM
Target Synonyms: COVID 19 IgM,N protein IgM
Tested Sample: Serum and plasma
Test Principale: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.
Specificity: This kit recognizes Human SARS-CoV-2 Nucleocapsid Protein IgM in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Nucleocapsid Protein IgM and analogues was observed.
Application: This ELISA kit applies to the in vitro qualitative determination of Human SARS-CoV-2 Nucleocapsid Protein IgM concentrations in Serum and plasma.
Research Area: SARS-CoV-2, COVID 19
Storage : An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
